Stem Cell Technologies in Neuroscience

Stem Cell Technologies in Neuroscience, edited by Amit K. Srivastava, Evan Y. Snyder and Yang D. Teng, is a specialized reference in the Neuromethods series focusing on stem cell technologies and their applications in neuroscience research. Published by Humana Press / Springer, this volume provides detailed laboratory protocols and practical methodologies for researchers working in stem cell biology, neuroscience and regenerative medicine.

The book covers techniques for the isolation, maintenance, differentiation and characterization of neural stem cells, along with experimental models of neurological diseases. It also provides protocols for stem cell genetic engineering, transplantation, donor-cell imaging, cryopreservation, extracellular vesicle studies and establishing a stem cell laboratory, making it a valuable resource for academic and translational research.

Key Features

• Part of the established Neuromethods series, Volume 126.
• Provides detailed laboratory protocols for stem cell research in neuroscience.
• Covers isolation, maintenance, differentiation and characterization of neural stem cells.
• Includes methods for developing in vitro stem cell-based neurological disease models.
• Describes three-dimensional cultures of human neural stem cells for disease modelling.
• Covers neural stem cell fate control using micropatterned substrates.
• Includes protocols for dopaminergic and GABAergic neuron differentiation from embryonic stem cells.
• Describes transfection methods for cultured primary neurons.
• Includes reprogramming of mouse fibroblasts into induced oligodendrocyte progenitor cells.
• Covers isolation and culture of oligodendrocyte precursor cells.
• Includes Schwann cell isolation and culture techniques.
• Describes generation of cerebral organoids from human pluripotent stem cells.
• Includes development of mouse cell-based in vitro blood-brain barrier models.
• Covers microfluidic approaches for studying traumatic brain injury.
• Includes neural stem cell protocols for experimental spinal cord injury research.
• Describes MRI-based approaches for studying stem cell delivery to the central nervous system.
• Includes standardized cryopreservation of stem cells.
• Covers isolation and characterization of extracellular vesicles in stem cell research.
• Provides essential considerations for establishing a new stem cell laboratory.
• Features step-by-step methodological information designed to support reproducible experimental work.

Ideal For

• Neuroscience researchers
• Stem cell researchers
• Regenerative medicine researchers
• Neurobiology laboratories
• Academic and translational researchers
• Life science graduate students
• Biomedical research students
• Biotechnology researchers
• Scientists working on neurological disease models
• Researchers studying neural stem cells and neuroregeneration
• Research laboratories establishing stem cell facilities

Buy Stem Cell Technologies in Neuroscience for detailed, protocol-based guidance on applying stem cell technologies to neuroscience research. This Neuromethods volume is a valuable laboratory reference for researchers and graduate students working in neural stem cells, neurological disease modelling, regenerative medicine and experimental neuroscience.